Anti-angiogenic activity of methanol extract of Phellinus linteus and its fractions

J Ethnopharmacol. 2010 Aug 19;131(1):56-62. doi: 10.1016/j.jep.2010.05.064. Epub 2010 Jun 8.

Abstract

Aim of the study: The aim of the present study was to investigate the effects of MeOH extract of PL (PLME) and its fractions on angiogenesis.

Materials and methods: PLME and its subsequent fractions (methylene chloride, ethyl acetate, n-butanol and aqueous fractions) were evaluated in vitro. Specifically, the anti-angiogenic activities of PLME and its fractions were investigated by measuring their effects on the proliferation, migration, tube formation and phosphorylation of vascular endothelial growth factor receptor (VEGFR)-2 in human umbilical vein endothelial cells (HUVECs). In addition, the in vivo Matrigel plug model was applied to evaluate new vessel formation.

Results: The results revealed that PLME and its subsequent fractions, except for the aqueous fraction, led to significant inhibition of the proliferation, migration, tube formation and VEGFR-2 phosphorylation of HUVECs as well as in vivo angiogenesis.

Conclusions: These findings indicate the potential for the use of PLME in pathological situations involving stimulated angiogenesis, such as inflammation and tumor development.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Agaricales
  • Angiogenesis Inhibitors / isolation & purification
  • Angiogenesis Inhibitors / pharmacology*
  • Animals
  • Cell Movement / drug effects
  • Cell Movement / physiology
  • Cell Proliferation / drug effects*
  • Cells, Cultured
  • Endothelial Cells / drug effects
  • Endothelial Cells / physiology
  • Endothelium, Vascular / drug effects
  • Endothelium, Vascular / physiology
  • Humans
  • Male
  • Methanol / pharmacology*
  • Mice
  • Mice, Inbred ICR
  • Phellinus
  • Plant Extracts / isolation & purification
  • Plant Extracts / pharmacology*
  • Polysaccharides / isolation & purification
  • Polysaccharides / pharmacology*
  • Vascular Endothelial Growth Factor Receptor-2 / antagonists & inhibitors
  • Vascular Endothelial Growth Factor Receptor-2 / metabolism

Substances

  • Angiogenesis Inhibitors
  • Phellinus linteus extract
  • Plant Extracts
  • Polysaccharides
  • Vascular Endothelial Growth Factor Receptor-2
  • Methanol