Qualitative study aimed at knowing the representations of mothers about the hospitalization of their premature son in a Neonatal Intensive Care Unit (NICU). The sample was composed of eighteen mothers of premature newborns hospitalized in a school hospital in Natal, Brazil. A semi-structured interview was used and data analysis showed that hospitalization of a child is represented by the mothers as meanings, feelings, difficulties and uncertainties. It is considered that prematurity represents a maternal challenge from the moment of the premature birth, proceeding with disturbances inherent to hospitalization. These require awareness, listening and dialogue on the part of health professionals in an effective manner in order to strengthen maternal adaptation to prematurity.