Generation of single-chain Fvs against detergent-solubilized recombinant antigens with a simple coating procedure

Torahiko Tanaka; Yuichiro Hasegawa; Makoto Saito; Masanori Ikeda; Nobuyuki Kato

doi:10.1016/j.jbiosc.2010.03.014

Generation of single-chain Fvs against detergent-solubilized recombinant antigens with a simple coating procedure

J Biosci Bioeng. 2010 Sep;110(3):374-6. doi: 10.1016/j.jbiosc.2010.03.014.

Authors

Torahiko Tanaka¹, Yuichiro Hasegawa, Makoto Saito, Masanori Ikeda, Nobuyuki Kato

Affiliation

¹ Department of Advanced Medical Science, Nihon University School of Medicine, 30-1 Oyaguchi-kamimachi, Itabashi, Tokyo 173-8610, Japan. tanakat@med.nihon-u.ac.jp

PMID: 20547361
DOI: 10.1016/j.jbiosc.2010.03.014

Abstract

Antigen coating on polystyrene is prevented by detergent. We present here a simple procedure to coat detergent-solubilized antigen for subsequent panning selection of single-chain Fv (scFv), the target antigen of which was the hepatitis C virus (HCV) non-structural protein (NS) 4B, an integral membrane protein.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Antigens / chemistry*
Detergents / chemistry*
Recombinant Proteins / chemistry*
Single-Chain Antibodies / biosynthesis*
Single-Chain Antibodies / chemistry*
Single-Chain Antibodies / genetics
Solubility

Substances

Antigens
Detergents
Recombinant Proteins
Single-Chain Antibodies