[Inhibition of growth and proliferation of Hep-2 cells by targeting hTERT gene using small interference RNA technology]

Lin Chuang Er Bi Yan Hou Tou Jing Wai Ke Za Zhi. 2010 Apr;24(7):308-10.
[Article in Chinese]

Abstract

Objective: To investigate the inhibition of hTERT gene to Hep-2 cells of laryngeal neoplasms.

Method: siRNA-hTERT mRNA was designed. Hep-2 cells, cultured in vitro, were transfected with lipofectamine 2000 and the inhibitory effect was detected by reverse transcriptase (RT-PCR), MTT assay, and morphology.

Result: 1. siRNA were transfected into Hep-2 cells successfully; 2. RT-PCR showed the exogenous hTERT was knocked down 70% by siRNA; 3. The morphology and the MTT result showed the hTERT siRNA to be able effectively to suppress the Hep-2 cell multiplication, the suppression rate achieves 65%.

Conclusion: siRNA target to hTERT can remarkably suppress the proliferation of Hep-2 cell.

Publication types

  • English Abstract
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carcinoma, Squamous Cell / genetics*
  • Cell Line, Tumor
  • Gene Targeting
  • Genetic Vectors
  • Humans
  • Laryngeal Neoplasms / genetics*
  • RNA / genetics
  • RNA, Messenger / genetics
  • RNA, Small Interfering*
  • Telomerase / genetics*
  • Transfection

Substances

  • RNA, Messenger
  • RNA, Small Interfering
  • RNA
  • hTERT mp30-38 peptide
  • Telomerase