Fluorescence enhancement of single DNA molecules confined in Si/SiO2 nanochannels

Lab Chip. 2010 Aug 21;10(16):2049-51. doi: 10.1039/c004878j. Epub 2010 Jun 14.

Abstract

We demonstrate that the detected emission intensity from YOYO-labeled DNA molecules confined in 180 nm deep Si/SiO2 nanofunnels changes significantly and not monotonically with the width of the funnel. This effect may be of importance for quantitative fluorescence microscopy and for experiments with a tight photon budget.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacteriophage lambda / genetics
  • Benzoxazoles / chemistry*
  • DNA / chemistry*
  • DNA, Viral / chemistry
  • Lab-On-A-Chip Devices
  • Microscopy, Fluorescence / methods*
  • Nanotechnology / instrumentation
  • Nanotechnology / methods*
  • Quinolinium Compounds / chemistry*
  • Silicon Dioxide / chemistry*

Substances

  • Benzoxazoles
  • DNA, Viral
  • Quinolinium Compounds
  • 1,1'-((4,4,7,7-tetramethyl)-4,7-diazaundecamethylene)bis-4-(3-methyl-2,3-dihydro(benzo-1,3-oxazole)-2-methylidene)quinolinium
  • Silicon Dioxide
  • DNA