Human mitochondrial mTERF wraps around DNA through a left-handed superhelical tandem repeat

Nat Struct Mol Biol. 2010 Jul;17(7):891-3. doi: 10.1038/nsmb.1859. Epub 2010 Jun 13.

Abstract

The regulation of mitochondrial DNA (mtDNA) processes is slowly being characterized at a structural level. We present here crystal structures of human mitochondrial regulator mTERF, a transcription termination factor also implicated in replication pausing, in complex with double-stranded DNA oligonucleotides containing the tRNA(Leu)(UUR) gene sequence. mTERF comprises nine left-handed helical tandem repeats that form a left-handed superhelix, the Zurdo domain.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Basic-Leucine Zipper Transcription Factors / chemistry*
  • Basic-Leucine Zipper Transcription Factors / metabolism*
  • DNA / chemistry
  • DNA / metabolism*
  • DNA, Mitochondrial / chemistry
  • DNA, Mitochondrial / metabolism
  • Humans
  • Mitochondria / chemistry
  • Mitochondria / metabolism
  • Mitochondrial Proteins
  • Models, Molecular
  • Protein Conformation
  • Tandem Repeat Sequences*

Substances

  • Basic-Leucine Zipper Transcription Factors
  • DNA, Mitochondrial
  • MTERF1 protein, human
  • Mitochondrial Proteins
  • DNA

Associated data

  • PDB/3N6S
  • PDB/3N7Q