Rapid detection of vancomycin-resistant enterococci from rectal swabs by the Cepheid Xpert vanA/vanB assay

Nancy Bourdon; Raphaël Bérenger; Romain Lepoultier; Audrey Mouet; Claire Lesteven; France Borgey; Marguerite Fines-Guyon; Roland Leclercq; Vincent Cattoir

doi:10.1016/j.diagmicrobio.2010.02.009

Rapid detection of vancomycin-resistant enterococci from rectal swabs by the Cepheid Xpert vanA/vanB assay

Diagn Microbiol Infect Dis. 2010 Jul;67(3):291-3. doi: 10.1016/j.diagmicrobio.2010.02.009.

Authors

Nancy Bourdon¹, Raphaël Bérenger, Romain Lepoultier, Audrey Mouet, Claire Lesteven, France Borgey, Marguerite Fines-Guyon, Roland Leclercq, Vincent Cattoir

Affiliation

¹ Centre National de la Résistance aux Antibiotiques, Laboratoire associé aux Entérocoques, Service de Microbiologie, C.H.U. Côte de Nacre, Caen, France.

PMID: 20542208
DOI: 10.1016/j.diagmicrobio.2010.02.009

Abstract

The detection of vancomycin-resistant enterococci using a novel commercial multiplex real-time polymerase chain reaction assay (Xpert vanA/vanB, Cepheid, Sunnyvale, CA) was evaluated on 804 rectal swab specimens. Compared to enriched culture, sensitivity and negative predictive value of this method were 100%. Many false-positive results were recovered (sensitivity, 85.4%; positive predictive value, 8.7%), especially for the vanB gene.

Publication types

Evaluation Study

MeSH terms

Bacterial Proteins / genetics*
Carbon-Oxygen Ligases / genetics*
Enterococcus / drug effects*
Enterococcus / genetics
Enterococcus / isolation & purification*
False Positive Reactions
Humans
Microbial Sensitivity Tests / methods
Molecular Diagnostic Techniques / methods*
Predictive Value of Tests
Rectum / microbiology*
Sensitivity and Specificity
Vancomycin Resistance*

Substances

Bacterial Proteins
VanA ligase, Bacteria
VanB protein, Enterococcus
Carbon-Oxygen Ligases