Penicillin biosynthesis in Aspergillus oryzae and its overproduction by genetic engineering

Junichiro Marui; Sumiko Ohashi-Kunihiro; Tomohiro Ando; Marie Nishimura; Hideaki Koike; Masayuki Machida

doi:10.1016/j.jbiosc.2010.01.001

Penicillin biosynthesis in Aspergillus oryzae and its overproduction by genetic engineering

J Biosci Bioeng. 2010 Jul;110(1):8-11. doi: 10.1016/j.jbiosc.2010.01.001. Epub 2010 Feb 4.

Authors

Junichiro Marui¹, Sumiko Ohashi-Kunihiro, Tomohiro Ando, Marie Nishimura, Hideaki Koike, Masayuki Machida

Affiliation

¹ Research Institute for Cell Engineering, National Institute of Advanced Industrial Science and Technology, Higashi 1-1-1, AIST Tsukuba Central 6, Tsukuba 305-8566, Ibaraki, Japan.

PMID: 20541108
DOI: 10.1016/j.jbiosc.2010.01.001

Abstract

Aspergillus oryzae penicillin biosynthetic genes were clustered. The penicillin production was positively regulated by VeA, a global gene regulator required for transcriptional expression of the penicillin biosynthetic genes. Overexpression of the biosynthetic genes by a strong promoter yielded a greater than 100-fold increase in penicillin production.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Aspergillus oryzae / genetics*
Aspergillus oryzae / metabolism*
Genes, Bacterial / genetics*
Genes, Regulator / genetics
Genetic Engineering*
Penicillins / biosynthesis*
Recombinant Proteins / genetics

Substances

Penicillins
Recombinant Proteins