A novel multiplex PCR method for Clostridium botulinum neurotoxin type A gene cluster typing

Kaoru Umeda; Yoshiyuki Seto; Tomoko Kohda; Masafumi Mukamoto; Shunji Kozaki

doi:10.1111/j.1348-0421.2010.00213.x

A novel multiplex PCR method for Clostridium botulinum neurotoxin type A gene cluster typing

Microbiol Immunol. 2010 May;54(5):308-12. doi: 10.1111/j.1348-0421.2010.00213.x.

Authors

Kaoru Umeda¹, Yoshiyuki Seto, Tomoko Kohda, Masafumi Mukamoto, Shunji Kozaki

Affiliation

¹ Department of Microbiology, Osaka City Institute of Public Health and Environmental Sciences, Tennoji-ku, Osaka, Japan.

PMID: 20536728
DOI: 10.1111/j.1348-0421.2010.00213.x

Abstract

A rapid, simple and sensitive multiplex PCR method for boNT/A gene cluster typing was developed by combining the results of BoNT/A subtype (boNT/A1 or /A2) gene detection with ha33 and/or p47 gene detection. Ten isolates associated with infant botulism in Japan were examined and divided into boNT/A gene cluster types 2 and 3 by origin (honey feeding or not) and period (1986-1987 or 1999-2007). It is suggested that this multiplex PCR method will be be useful for epidemiological studies of botulism.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacterial Typing Techniques / methods
Botulinum Toxins, Type A / genetics*
Botulism / microbiology*
Clostridium botulinum type A / genetics*
DNA, Bacterial / genetics
Genotype
Humans
Infant
Multigene Family
Polymerase Chain Reaction / methods

Substances

DNA, Bacterial
Botulinum Toxins, Type A