Pyrene-functionalized triazole-linked 2'-deoxyuridines-probes for discrimination of single nucleotide polymorphisms (SNPs)

Michael E Østergaard; Dale C Guenther; Pawan Kumar; Bharat Baral; Lee Deobald; Andrzej J Paszczynski; Pawan K Sharma; Patrick J Hrdlicka

doi:10.1039/c0cc01133a

Pyrene-functionalized triazole-linked 2'-deoxyuridines-probes for discrimination of single nucleotide polymorphisms (SNPs)

Chem Commun (Camb). 2010 Jul 21;46(27):4929-31. doi: 10.1039/c0cc01133a. Epub 2010 Jun 4.

Authors

Michael E Østergaard¹, Dale C Guenther, Pawan Kumar, Bharat Baral, Lee Deobald, Andrzej J Paszczynski, Pawan K Sharma, Patrick J Hrdlicka

Affiliation

¹ Dept. of Chemistry, Univ. of Idaho, Moscow, ID-83844, USA.

PMID: 20526503
DOI: 10.1039/c0cc01133a

Abstract

Oligonucleotides modified with pyrene-functionalized triazole-linked 2'-deoxyuridines display remarkable hybridization-induced increases in fluorescence emission and enable efficient fluorescent discrimination of SNPs via G-specific quenching.

Publication types

Research Support, Non-U.S. Gov't
Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

DNA Probes / chemistry*
Deoxyuridine / chemistry*
Nucleic Acid Hybridization
Oligonucleotides / chemistry
Oligonucleotides / metabolism
Polymorphism, Single Nucleotide*
Pyrenes / chemistry*
Spectrometry, Fluorescence
Triazoles / chemistry*

Substances

DNA Probes
Oligonucleotides
Pyrenes
Triazoles
Deoxyuridine