[Development and clinical evaluation of a new urinary protein test paper (URINE-TP)]

Rinsho Byori. 1991 Apr;39(4):398-404.
[Article in Japanese]

Abstract

Because standard urinary protein test paper reacts more strongly to albumin than to globulin, we attempted to develop a test paper sensitive to globulin by applying chromatic analysis principles used in quantitative analysis of urinary protein. We developed a new urinary protein test paper (URINE-TP) containing a reagent of ACID VIOLET 17. It was found that URINE-TP reacts with the same strength to gamma-globulin as to albumin. This new test paper therefore allows accurate detection of Bence Jones protein, which was formerly detectable with standard test paper. There was a correlation between values for urinary protein obtained with URINE-TP and those obtained by quantitative analysis. As with standard test paper, URINE-TP indicates urinary protein values in the normal range by a lack of reaction, but on URINE-TP positive and negative results are more clearly distinguishable than on standard test paper. From these results, we conclude that URINE-TP enables detection of both gamma-globulin and albumin in the urine, and that is as sensitive as the analytical method in the screening of urinary proteins.

Publication types

  • English Abstract

MeSH terms

  • Bence Jones Protein / urine
  • Globulins / urine
  • Humans
  • Proteinuria / diagnosis*
  • Reagent Strips
  • Rosaniline Dyes*

Substances

  • Globulins
  • Reagent Strips
  • Rosaniline Dyes
  • violet BNP
  • Bence Jones Protein