Evaluation of a commercial microarray system for detection of SHV-, TEM-, CTX-M-, and KPC-type beta-lactamase genes in Gram-negative isolates

J Clin Microbiol. 2010 Jul;48(7):2618-22. doi: 10.1128/JCM.00568-10. Epub 2010 May 26.

Abstract

We evaluated the ability of a commercial microarray system (Check KPC/ESBL; Check-Points Health BV) to detect clinically important class A beta-lactamase genes. A total of 106 Gram-negative strains were tested. The following sensitivity and specificity results were recorded, respectively: for bla(SHV), 98.8% and 100%; for bla(TEM), 100% and 96.4%; and for bla(CTX-M) and bla(KPC), 100% and 100%.

Publication types

  • Comparative Study
  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Bacterial Proteins / genetics*
  • DNA, Bacterial / chemistry
  • Evaluation Studies as Topic
  • Gram-Negative Bacteria / genetics*
  • Gram-Negative Bacterial Infections / microbiology
  • Humans
  • Oligonucleotide Array Sequence Analysis / methods*
  • Polymerase Chain Reaction / methods
  • Sensitivity and Specificity
  • Sequence Analysis, DNA / methods*
  • beta-Lactamases / genetics*

Substances

  • Bacterial Proteins
  • DNA, Bacterial
  • beta-Lactamases