Over the past two decades, poly(N-isopropyl acrylamide) (pNIPAM) has become widely used for bioengineering applications. In particular, pNIPAM substrates have been used for the nondestructive release of biological cells and proteins. In this feature article, we review the applications for which pNIPAM substrates have been used to release biological cells, including for the study of the extracellular matrix (ECM), for cell sheet engineering and tissue transplantation, the formation of tumorlike spheroids, the study of bioadhesion and bioadsorption, and the manipulation or deformation of individual cells. The articles reviewed include submissions from our own group as well as from those performing research in the field worldwide.