Analysis of 14 highly informative SNP markers on X chromosome by TaqMan SNP genotyping assay

Li Li; Chengtao Li; Suhua Zhang; Shumin Zhao; Yan Liu; Yuan Lin

doi:10.1016/j.fsigen.2010.04.004

Analysis of 14 highly informative SNP markers on X chromosome by TaqMan SNP genotyping assay

Forensic Sci Int Genet. 2010 Oct;4(5):e145-8. doi: 10.1016/j.fsigen.2010.04.004. Epub 2010 May 20.

Authors

Li Li¹, Chengtao Li, Suhua Zhang, Shumin Zhao, Yan Liu, Yuan Lin

Affiliation

¹ Shanghai Key Laboratory of Forensic Medicine, Institute of Forensic Science, Ministry of Justice, PR China, Shanghai 200063, China. anneinchina@163.com

PMID: 20493796
DOI: 10.1016/j.fsigen.2010.04.004

Abstract

Objective: To genotype X-linked single nucleotide polymorphisms by TaqMan SNP genotyping assay.

Methods: 14 SNP markers on X chromosome were selected and genotyped by TaqMan SNP genotyping assay in Chinese Han population samples.

Results: According to the results of population studies, deviations from Hardy-Weinberg equilibrium could be found at rs1299087 SNP loci, no deviations from Hardy-Weinberg equilibrium could be found at the other 13 SNP loci. The 14 X-SNPs were high informative. The overall power of discrimination (CPD) in females and in males were 0.999998379 and 0.999899, respectively. The combined exclusion probability in paternity testing for trios and duos were 0.9983 and 0.9788, respectively.

Conclusion: SNP typing by TaqMan SNP genotyping assay is suitable for low-throughput application. The forensic efficiency parameters showed that the 14 X-linked SNPs are highly informative and seem to be useful for forensic genetics except rs1299087.

Publication types

Research Support, Non-U.S. Gov't
Validation Study

MeSH terms

Chromosomes, Human, X*
Female
Genetic Markers*
Genotype
Humans
Male
Polymorphism, Single Nucleotide*

Substances

Genetic Markers