Human cytomegalovirus-specific CD4(+) and CD8(+) T-cell response determination: comparison of short-term (24h) assays vs long-term (7-day) infected dendritic cell assay in the immunocompetent and the immunocompromised host

Clin Immunol. 2010 Aug;136(2):269-81. doi: 10.1016/j.clim.2010.04.008. Epub 2010 May 20.

Abstract

Human cytomegalovirus (HCMV)-specific CD4(+) and CD8(+) T-cells were measured in the immunocompetent host as well as in 13 solid-organ transplant recipients (SOTR), and 12 young hematopoietic stem cell transplant recipients (HSCTR) by using a long-term (7-day) assay based on PBMC stimulation by HCMV-infected dendritic cells (iDC), and two short-term (24h) assays, one for CD4(+) stimulation by infected cell lysate (iCL), and the other for CD8(+) stimulation by a pool of 34 epitopic peptides (pep-pool). In the immunocompetent, the number of T-cells activated by either iCL or the pep-pool was significantly reduced with respect to iDC. In both SOTR and HSCTR, the number of T-cells activated by iDC was comparable to that activated by iCL or the pep-pool. A significant correlation between iDC-activated T-cells and T-cells activated by either iCL or the pep-pool was observed. In conclusion, whenever a rapid result is needed, short-term assays may efficiently replace the iDC assay.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Aged
  • CD4-Positive T-Lymphocytes / physiology*
  • CD8-Positive T-Lymphocytes / physiology*
  • Child
  • Child, Preschool
  • Cytomegalovirus / immunology*
  • Cytomegalovirus Infections / immunology
  • Dendritic Cells / immunology
  • Female
  • Hematopoietic Stem Cell Transplantation
  • Humans
  • Immunoassay / methods*
  • Immunocompetence / immunology*
  • Immunocompromised Host*
  • Infant
  • Male
  • Middle Aged
  • Organ Transplantation
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Time Factors
  • Young Adult