Identification of X chromosome copies by quantitative real-time polymerase chain reaction for population screening tests

Ester S Ramos; Juliana V Serafim; Paula L Takeuchi; Cintia R Marcondes; Adriane Araújo

doi:10.1016/j.fertnstert.2010.04.008

Identification of X chromosome copies by quantitative real-time polymerase chain reaction for population screening tests

Fertil Steril. 2010 Nov;94(6):2476-8. doi: 10.1016/j.fertnstert.2010.04.008. Epub 2010 May 20.

Authors

Ester S Ramos¹, Juliana V Serafim, Paula L Takeuchi, Cintia R Marcondes, Adriane Araújo

Affiliation

¹ Department of Genetics, Faculty of Medicine of Ribeirão Preto, University of São Paulo, Ribeirão Preto, São Paulo, Brazil. esramos@rge.fmrp.usp.br

PMID: 20493475
DOI: 10.1016/j.fertnstert.2010.04.008

Abstract

We adapted a method that allows the identification of X chromosome copy number by quantitative real-time polymerase chain reaction (PCR) in a short amount of time. It may be used for sex and aneuploidy involving X chromosome large population screening tests.

Publication types

Research Support, Non-U.S. Gov't
Validation Study

MeSH terms

Aneuploidy*
Case-Control Studies
Chromosome Duplication
Chromosomes, Human, X* / genetics
Female
Gene Dosage
Genetic Testing / methods*
Genetics, Population / methods
Humans
Male
Polymerase Chain Reaction / methods*
Sex Chromosome Aberrations
Sex Chromosome Disorders / diagnosis
Sex Chromosome Disorders / genetics