Modified siRNAs for the study of the PAZ domain

Alvaro Somoza; Montserrat Terrazas; Ramón Eritja

doi:10.1039/c003221b

Modified siRNAs for the study of the PAZ domain

Chem Commun (Camb). 2010 Jun 28;46(24):4270-2. doi: 10.1039/c003221b. Epub 2010 May 19.

Authors

Alvaro Somoza¹, Montserrat Terrazas, Ramón Eritja

Affiliation

¹ IMDEA-Nanociencia, 28049, Madrid, Spain. alvaro.somoza@imdea.org

PMID: 20485810
DOI: 10.1039/c003221b

Abstract

Chemical modifications aimed at stabilizing the interaction between the 3'-end of siRNAs and the PAZ domain of RISC have been tested for their effect on RNAi activity. Such modifications contribute positively to the stability of siRNAs in human serum.

MeSH terms

Cell Line, Tumor
HeLa Cells
Humans
Protein Binding
Protein Structure, Tertiary
RNA Interference
RNA, Small Interfering / chemistry*
RNA-Induced Silencing Complex / chemistry*

Substances

RNA, Small Interfering
RNA-Induced Silencing Complex