Abstract
Previously, we showed that the Shiga toxin type 2 (Stx2)-expressing Escherichia coli O157:H7 strain 86-24 colonized mice better than did its isogenic stx(2) negative mutant. Here, we confirmed that finding by demonstrating that Stx2 given orally to mice increased the levels of the 86-24 stx(2) mutant shed in feces. Then we assessed the impact of Stx2-neutralizing antibodies, administered passively or generated by immunization with an Stx2 toxoid, on E. coli O157:H7 colonization of mice. We found that such antibodies reduced the E. coli O157:H7 burden in infected mice and, as anticipated, also protected them from weight loss and death.
Published by Elsevier Ltd.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antibodies, Bacterial / immunology*
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Antibodies, Neutralizing / immunology*
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Enzyme-Linked Immunosorbent Assay
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Escherichia coli Infections / immunology
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Escherichia coli Infections / microbiology*
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Escherichia coli Infections / prevention & control*
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Escherichia coli O157 / genetics*
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Escherichia coli O157 / immunology*
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Feces / chemistry
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Female
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Immunization, Passive
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Mice
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Mice, Inbred BALB C
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Neutralization Tests
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Rabbits
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Shiga Toxin 2 / genetics
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Shiga Toxin 2 / immunology*
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Toxoids / immunology
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Toxoids / therapeutic use
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Vaccination
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Weight Loss
Substances
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Antibodies, Bacterial
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Antibodies, Neutralizing
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Shiga Toxin 2
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Toxoids