Wiring of pyranose dehydrogenase with osmium polymers of different redox potentials

Muhammad Nadeem Zafar; Federico Tasca; Susan Boland; Magdalena Kujawa; Ilabahen Patel; Clemens K Peterbauer; Dónal Leech; Lo Gorton

doi:10.1016/j.bioelechem.2010.04.002

Wiring of pyranose dehydrogenase with osmium polymers of different redox potentials

Bioelectrochemistry. 2010 Nov;80(1):38-42. doi: 10.1016/j.bioelechem.2010.04.002. Epub 2010 Apr 22.

Authors

Muhammad Nadeem Zafar¹, Federico Tasca, Susan Boland, Magdalena Kujawa, Ilabahen Patel, Clemens K Peterbauer, Dónal Leech, Lo Gorton

Affiliation

¹ Department of Analytical Chemistry, Lund University, PO Box 124, SE-221 00 Lund, Sweden.

PMID: 20466600
DOI: 10.1016/j.bioelechem.2010.04.002

Abstract

In this study, five different flexible osmium based redox polymers were investigated for their ability to efficiently "wire" the oxidoreductase pyranose dehydrogenase (PDH, EC 1.1.99.29) from Agaricus meleagris, on graphite electrodes for possible applications in biofuel cells. A series of newly synthesised osmium based redox polymers covering the potential range between -270 and +160 mV vs. Ag|AgCl (0.1M KCl) was used. The performance of the redox polymers for enzyme wiring was investigated using glucose as substrate. The optimal operational conditions such as pH and potential were investigated.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Agaricus / enzymology
Biosensing Techniques / instrumentation
Biosensing Techniques / methods*
Carbohydrate Dehydrogenases / chemistry*
Carbohydrate Dehydrogenases / metabolism
Electrochemistry
Electrodes
Glucose / chemistry
Glucose / metabolism
Graphite / chemistry
Hydrogen-Ion Concentration
Organometallic Compounds / chemistry*
Osmium / chemistry*
Oxidation-Reduction
Polymers / chemistry*
Silver / chemistry
Silver Compounds / chemistry

Substances

Organometallic Compounds
Polymers
Silver Compounds
Osmium
Silver
Graphite
Carbohydrate Dehydrogenases
Glucose
silver chloride