Metallo-beta-lactamase-producing imipenem-resistant Pseudomonas aeruginosa clinical isolates in a university teaching hospital in Malaysia: detection of IMP-7 and first identification of IMP-4, VIM-2, and VIM-11

Diagn Microbiol Infect Dis. 2010 Jul;67(3):294-6. doi: 10.1016/j.diagmicrobio.2010.02.010. Epub 2010 May 11.

Abstract

Ninety (n = 90) imipenem-resistant Pseudomonas aeruginosa (IRPA) clinical isolates collected randomly during 2005 to 2008 from University Malaya Medical Center were assessed for the presence of different variants of metallo-beta-lactamase (MBL) genes. Polymerase chain reaction (PCR) assay detected 32 (n = 32) MBL gene PCR-positive isolates with the presence of bla(IMP) gene in 14 (n = 14) and bla(VIM) in 18 (n = 18) isolates. Four allelic variants, bla(IMP-7) (12 isolates), bla(IMP-4) (2 isolates), bla(VIM-2) (17 isolates), and bla(VIM-11) (1 isolate), of MBL genes were identified. This study is the first report of detection of bla(IMP-4), bla(VIM-2), and bla(VIM-11) MBL genes from IRPA clinical isolates in Malaysia.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Anti-Bacterial Agents / pharmacology*
  • Bacterial Proteins / genetics*
  • DNA, Bacterial / chemistry
  • DNA, Bacterial / genetics
  • Hospitals, University
  • Humans
  • Imipenem / pharmacology*
  • Malaysia
  • Molecular Sequence Data
  • Polymerase Chain Reaction
  • Pseudomonas Infections / microbiology
  • Pseudomonas aeruginosa / drug effects*
  • Pseudomonas aeruginosa / enzymology*
  • Pseudomonas aeruginosa / isolation & purification
  • Sequence Analysis, DNA
  • beta-Lactam Resistance*
  • beta-Lactamases / genetics*

Substances

  • Anti-Bacterial Agents
  • Bacterial Proteins
  • DNA, Bacterial
  • Imipenem
  • beta-Lactamases

Associated data

  • GENBANK/GQ221779
  • GENBANK/GQ221780
  • GENBANK/GQ221781