Objective: To study the inhibitory effect of Juhua Jueming powder (JJP) on high-risk corneal transplantation immune rejection in rats.
Methods: The high-risk corneal transplantation immune rejection rat model was established by inducing corneal neoangiogenesis by suture method and the penetrating transplantation. Model rats were divided into two groups, the treated group and the control group, they were administered with JJP 0.1 g (dissolved in 2 mL water) and normal saline respectively via gastric infusion every day after transplantation. The survival status of the allograft, histopathology, local and systemic immune status in the recipients were observed using immunofluorescence histochemistry and flow cytometry.
Results: The survival time of the allograft in the treated group (14.50 +/- 3.55 days) was significantly longer than that in the control group (8.25 +/- 0.71 days, P < 0.01). Levels of Fas, FasL expressions in iris were stronger, and the percentage of CD4 CD FOXP3 positive cells in peripheral blood was less (5.11 +/- 3. 92% vs. 14.81 +/- 2.58%) in the control group than those in the treated group respectively. The concentration of IL-2 was lower while that of IL-10 was higher in aqueous humor of the treated group than those of the control group, respectively.
Conclusion: JJP has certain effect for preventing high-risk corneal transplantation immune rejection in rat model.