Transaminations with isopropyl amine: equilibrium displacement with yeast alcohol dehydrogenase coupled to in situ cofactor regeneration

Karim Engelmark Cassimjee; Cecilia Branneby; Vahak Abedi; Andrew Wells; Per Berglund

doi:10.1039/c0cc00050g

Transaminations with isopropyl amine: equilibrium displacement with yeast alcohol dehydrogenase coupled to in situ cofactor regeneration

Chem Commun (Camb). 2010 Aug 14;46(30):5569-71. doi: 10.1039/c0cc00050g. Epub 2010 May 12.

Authors

Karim Engelmark Cassimjee¹, Cecilia Branneby, Vahak Abedi, Andrew Wells, Per Berglund

Affiliation

¹ Division of Biochemistry, School of Biotechnology, Royal Institute of Technology (KTH), AlbaNova University Centre, SE-106 91 Stockholm, Sweden.

PMID: 20461279
DOI: 10.1039/c0cc00050g

Abstract

Enantiopure chiral amines synthesis using omega-transaminases is hindered by an unfavourable equilibrium, but when using isopropylamine as the amine donor the equilibrium can be completely displaced by using a specific dehydrogenase in situ for removal of formed acetone.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Acetone / metabolism
Alcohol Dehydrogenase / metabolism*
Amination
Amines / chemical synthesis*
Amines / metabolism
Propylamines / metabolism*
Saccharomyces cerevisiae / enzymology*
Stereoisomerism

Substances

Amines
Propylamines
Acetone
Alcohol Dehydrogenase
2-propylamine