Abstract
"Sandwich-type" noncompetitive (immunometric) assays allow for high-sensitivity high-throughput macromolecule sensing and determination but cannot be used on small molecules (haptens). Here, we isolated single-chain Fv fragments from a phage-display library, which bound to complexes of particular haptens (vitamin D and A derivatives) with immobilized beta-cyclodextrin or beta-maltosyl residues, and formed ternary complexes. These scFvs enabled novel "semisandwich-type" immunometric assays of haptens with nanomole-range sensitivities.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Biosensing Techniques / methods*
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Carbohydrate Conformation
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Cattle
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Haptens / analysis
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Haptens / chemistry
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Haptens / immunology
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High-Throughput Screening Assays
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Immunoassay / methods*
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Models, Molecular
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Oligosaccharides / metabolism*
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Serum Albumin, Bovine / metabolism
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Single-Chain Antibodies / immunology
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Single-Chain Antibodies / metabolism
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beta-Cyclodextrins / chemistry
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beta-Cyclodextrins / metabolism
Substances
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Haptens
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Oligosaccharides
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Single-Chain Antibodies
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beta-Cyclodextrins
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Serum Albumin, Bovine