Detection of pandemic influenza A H1N1 virus by multiplex reverse transcription-PCR with a GeXP analyzer

Meng Qin; Da-yan Wang; Fang Huang; Kai Nie; Mei Qu; Miao Wang; Yue-long Shu; Xue-jun Ma

doi:10.1016/j.jviromet.2010.04.031

Detection of pandemic influenza A H1N1 virus by multiplex reverse transcription-PCR with a GeXP analyzer

J Virol Methods. 2010 Sep;168(1-2):255-8. doi: 10.1016/j.jviromet.2010.04.031. Epub 2010 May 7.

Authors

Meng Qin¹, Da-yan Wang, Fang Huang, Kai Nie, Mei Qu, Miao Wang, Yue-long Shu, Xue-jun Ma

Affiliation

¹ National Institute for Viral Disease Control and Prevention, Chinese Center for Disease Control and Prevention, Xuan Wu District, Beijing 100052, China.

PMID: 20452377
DOI: 10.1016/j.jviromet.2010.04.031

Abstract

A novel application of the GeXP genetic analysis system for the differential detection of pandemic influenza A H1N1 from seasonal influenza A H1N1 and H3N2 is described. The assay was evaluated using identified influenza viruses and clinical samples. The results indicate that the assay is both highly sensitive and specific for the detection of the pandemic influenza A H1N1 virus with a detection limit of 10 copies per reaction superior to that of assays in use currently. The assay is able to detect potential mixed infections. This technique has the potential to provide both a powerful method to enhance surveillance of influenza and a platform for investigating the differentiation of other similar pathogens.

Publication types

Evaluation Study
Research Support, Non-U.S. Gov't

MeSH terms

Disease Outbreaks
Humans
Influenza A Virus, H1N1 Subtype / genetics
Influenza A Virus, H1N1 Subtype / isolation & purification*
Influenza, Human / diagnosis*
Influenza, Human / epidemiology
Influenza, Human / virology*
Reverse Transcriptase Polymerase Chain Reaction / methods*
Sensitivity and Specificity
Virology / methods*