Detection of pathogenic Streptococcus suis bacteria using magnetic glycoparticles

Org Biomol Chem. 2010 May 21;8(10):2425-9. doi: 10.1039/c000819b. Epub 2010 Mar 22.

Abstract

Detection of the zoonotic bacterial pathogen Streptococcus suis was achieved using magnetic glycoparticles. The bacteria contain an adhesion protein for the carbohydrate sequence Galalpha1,4Gal. After incubation with various amounts of the pathogen, magnetic concentration and ATP detection, bacterial levels down to 10(5) cfu could be detected. Submicrometer particles were needed, since with the larger microparticles the method did not succeed.

MeSH terms

  • Adenosine Triphosphate / analysis
  • Bacterial Proteins / metabolism
  • Carbohydrate Metabolism
  • Carbohydrates / chemistry*
  • Luminescent Measurements
  • Magnetics*
  • Particle Size
  • Streptococcus suis / isolation & purification*
  • Streptococcus suis / metabolism

Substances

  • Bacterial Proteins
  • Carbohydrates
  • Adenosine Triphosphate