Substantial quantities of the high energy derivative oligophosphoglyceroyl-ATP are located in mitochondria in rat liver

B Patel; C D Gove; W L Hutchinson; J Mowbray

doi:10.1016/0304-4165(91)90058-o

Substantial quantities of the high energy derivative oligophosphoglyceroyl-ATP are located in mitochondria in rat liver

Biochim Biophys Acta. 1991 May 24;1074(1):178-81. doi: 10.1016/0304-4165(91)90058-o.

Authors

B Patel¹, C D Gove, W L Hutchinson, J Mowbray

Affiliation

¹ Department of Biochemistry, University College London, U.K.

PMID: 2043668
DOI: 10.1016/0304-4165(91)90058-o

Abstract

Perfusion with [8-14C]adenosine demonstrated the likely existence in rat liver of oligophosphoglyceroyl-ATP (OPG-ATP). Purification followed by assay with a new specific 3' phosphodiesterase confirmed this. The quantities present were 5-10-fold those found previously and comparable to total soluble nucleotides. OPG-ATP was also purified from the mitochondrial fraction, shown to co-distribute with succinate dehydrogenase and can be co-purified with an enzyme confined to intermembrane space.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adenosine Triphosphate / analogs & derivatives*
Adenosine Triphosphate / analysis
Animals
Chromatography, DEAE-Cellulose
Glyceric Acids / analysis*
Intracellular Membranes / chemistry
Male
Mitochondria, Liver / chemistry*
Organophosphorus Compounds / analysis*
Perfusion
Phenols
Rats
Rats, Inbred Strains
Succinate Dehydrogenase / analysis

Substances

Glyceric Acids
Organophosphorus Compounds
Phenols
oligophosphoglycerol-ATP
Adenosine Triphosphate
Succinate Dehydrogenase