A revisit to the one form kinetic model of prothrombinase

Biophys Chem. 2010 Jun;149(1-2):28-33. doi: 10.1016/j.bpc.2010.03.011. Epub 2010 Mar 25.

Abstract

Thrombin is generated enzymatically from prothrombin by two pathways with the intermediates of meizothrombin and prethrombin-2. Experimental concentration profiles from two independent groups for these two pathways have been re-analyzed. By rationally combining the independent data sets, a simple mechanism can be established and rate constants determined. A structural model is consistent with the data-derived finding that mechanisms that feature channeling or ratcheting are not necessary to describe thrombin production.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Blood Coagulation
  • Catalytic Domain
  • Kinetics
  • Molecular Dynamics Simulation*
  • Protein Structure, Tertiary
  • Prothrombin / chemistry
  • Prothrombin / metabolism
  • Thrombin / chemistry
  • Thrombin / metabolism
  • Thromboplastin / chemistry*
  • Thromboplastin / metabolism

Substances

  • Prothrombin
  • Thromboplastin
  • Thrombin