Obtaining mannanoligosaccharide preparations by means of the mechanoenzymatic hydrolysis of yeast biomass

Appl Biochem Biotechnol. 2010 Nov;162(7):2008-14. doi: 10.1007/s12010-010-8976-2. Epub 2010 Apr 29.

Abstract

A new method of obtaining biologically active mannanoligosaccharides is proposed. It involves mechanical activation of the enzymatic hydrolysis of components forming the supramolecular structure of the cell wall. Processes that take place during mechanical treatment and enzymatic hydrolysis of yeast biomass and lead to an increase in the availability of mannanoligosaccharides of the yeast cell walls are investigated. The efficiency of the use of mechanoenzymatic approach to obtaining mannanoligosaccharide preparations is evaluated.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Biomass
  • Cell Wall / chemistry
  • Cell Wall / metabolism
  • Cellulases / chemistry*
  • Fungal Proteins / chemistry*
  • Hydrolysis
  • Industrial Microbiology / methods*
  • Mannans / chemistry*
  • Mannans / isolation & purification
  • Mannans / metabolism
  • Mannose / analysis
  • Mannose / isolation & purification
  • Mannose / metabolism
  • Oligosaccharides / chemistry*
  • Oligosaccharides / isolation & purification
  • Oligosaccharides / metabolism
  • Saccharomyces cerevisiae / chemistry*
  • Saccharomyces cerevisiae / metabolism
  • Trichoderma / enzymology

Substances

  • Fungal Proteins
  • Mannans
  • Oligosaccharides
  • Cellulases
  • Mannose