Honeycomb films were prepared by using a photo-responsive amphiphilic copolymer containing photochromic spiropyran. Through the photo-responsivity of spiropyran, the molecular polarities provide solubility changes against chloroform vapor after UV irradiation. The merocyanine area formed with UV irradiation retained its honeycomb-pattern against chloroform vapor, though the spiropyran area without UV irradiation dissolved.