Oxygen-independent photonuclease activity of a new iron(II) complex

Bernardo de Souza; Fernando R Xavier; Rosely A Peralta; Adailton J Bortoluzzi; Gilmar Conte; Hugo Gallardo; Franciele L Fischer; Giselle Bussi; Hernán Terenzi; Ademir Neves

doi:10.1039/b922560a

Oxygen-independent photonuclease activity of a new iron(II) complex

Chem Commun (Camb). 2010 May 21;46(19):3375-7. doi: 10.1039/b922560a. Epub 2010 Mar 18.

Authors

Bernardo de Souza¹, Fernando R Xavier, Rosely A Peralta, Adailton J Bortoluzzi, Gilmar Conte, Hugo Gallardo, Franciele L Fischer, Giselle Bussi, Hernán Terenzi, Ademir Neves

Affiliation

¹ Departamento de Química, Universidade Federal de Santa Catarina, 88040-900, Florianópolis, SC, Brazil.

PMID: 20428519
DOI: 10.1039/b922560a

Abstract

A new iron(ii) complex was synthesized and its photonuclease activity against plasmid DNA was evaluated. After kinetic experiments a k(cat) of 18.86 +/- 2.9 h(-1) was obtained, one of the highest nuclease activities of synthetic metallonucleases, selectively activated by 365 nm UV light.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Crystallography, X-Ray
DNA / chemistry
DNA / drug effects*
DNA Cleavage
Deoxyribonucleases / chemistry
Deoxyribonucleases / metabolism
Ferrous Compounds / chemical synthesis
Ferrous Compounds / chemistry
Ferrous Compounds / pharmacology*
Ligands
Models, Molecular
Molecular Structure
Oxygen / chemistry*
Photolysis
Plasmids / chemistry
Ultraviolet Rays

Substances

Ferrous Compounds
Ligands
DNA
Deoxyribonucleases
Oxygen