An endogenous, systemic RNAi pathway in plants

EMBO J. 2010 May 19;29(10):1699-712. doi: 10.1038/emboj.2010.65. Epub 2010 Apr 22.

Abstract

Recent work on metazoans has uncovered the existence of an endogenous RNA-silencing pathway that functionally recapitulates the effects of experimental RNA interference (RNAi) used for gene knockdown in organisms such as Caenorhabditis elegans and Drosophila. The endogenous short interfering (si)RNA involved in this pathway are processed by Dicer-like nucleases from genomic loci re-arranged to form extended inverted repeats (IRs) that produce perfect or near-perfect dsRNA molecules. Although such IR loci are commonly detected in plant genomes, their genetics, evolution and potential contribution to plant biology through endogenous silencing have remained largely unexplored. Through an exhaustive analysis performed using Arabidopsis, we provide here evidence that at least two such endogenous IRs are genetically virtually indistinguishable from the transgene constructs commonly used for RNAi in plants. We show how these loci can be useful probes of the cellular mechanism and fluidity of RNA-silencing pathways in plants, and provide evidence that they may arise and disappear on an ecotype scale, show highly cell-specific expression patterns and respond to various stresses. IR loci thus have the potential to act as molecular sensors of the local environments found within distinct ecological plant niches. We further show that the various siRNA size classes produced by at least one of these IR loci are functionally loaded into cognate effector proteins and mediate both post-transcriptional gene silencing and RNA-directed DNA methylation (RdDM) of endogenous as well as exogenous targets. Finally, and as previously reported during plant experimental RNAi, we provide evidence that endogenous IR-derived siRNAs of all size classes are not cell-autonomous and can be transported through graft junctions over long distances, in target tissues where they are functional, at least in mediating RdDM. Collectively, these results define the existence of a bona fide, endogenous and systemic RNAi pathway in plants that may have implications in adaptation, epiallelism and trans-generational memory.

Publication types

  • Research Support, Non-U.S. Gov't
  • Retracted Publication

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Arabidopsis / genetics*
  • Arabidopsis Proteins / metabolism
  • Caenorhabditis elegans
  • Cell Cycle Proteins / metabolism
  • DNA Methylation
  • Drosophila
  • Gene Silencing
  • Molecular Sequence Data
  • Mutation
  • Plants / genetics*
  • RNA Interference*
  • RNA, Small Interfering / metabolism
  • Ribonuclease III / metabolism
  • Sequence Homology, Amino Acid
  • Transcription, Genetic
  • Transgenes

Substances

  • Arabidopsis Proteins
  • Cell Cycle Proteins
  • RNA, Small Interfering
  • DCL1 protein, Arabidopsis
  • Ribonuclease III