We have determined delta(13)C, delta(2)H and delta(18)O isotopic abundances in Andalusian olive oils. In addition, the fatty acid composition and the distribution of isomers at positions 1,3 and 2 of glycerol were determined by (1)H and (13)C nuclear magnetic resonance (NMR) spectroscopy, respectively. Isotopic results obtained for four series of oil samples extracted from olives harvested in the 2004/05 and 2005/06 seasons are discussed in terms of olive variety, ripeness, geographical origin, fatty acid composition and growing altitude. A distinction was also established between olives grown in irrigated and in dry land by studying selected samples of the previous series and others from the 2005/06, 2006/07, 2007/08 and 2008/09 seasons. The results showed that olive ripeness does not influence the abundance of any of the three isotopes studied. On the other hand, the olive variety influences the abundance of the oxygen and hydrogen isotopes, and also, less markedly, that of carbon. No clear-cut effect of height or latitude on isotope values is observed, probably because the olive variety also changes with height and latitude, thus masking such influences. The oil samples from dryland-grown olives had increased delta(13)C values relative to irrigation-grown olives. In addition, no definite relationship appears to exist between isotope distribution and fatty acid composition. Finally, oil samples from olives harvested in the 2005/06 season in Italy could be distinguished from those from Spain in terms of their isotopic values (delta(2)H mainly).
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