Preparation of hydroxyapatite from animal bones

Acta Bioeng Biomech. 2009;11(4):23-8.

Abstract

This paper presents the method of obtaining hydroxyapatite from animal bones. Bone sludge and calcined products were characterized by X-ray diffraction (XRD), Fourier transformed infrared spectroscopy (FT-IR) and scanning electron microscopy (SEM). Calcium concentration was determined with titration, and phosphorus--spectrophotometrically. Making use of the AAS and ICP methods the content of microelements was determined. In all the products, hydroxyapatite was the only crystalline phase indicated. The FT-IR spectra confirmed that calcination removed the total of organic substances. Calcium and phosphorus contents were 38% and 18%, respectively, which corresponded to the Ca/P molar ratio of nonstoichiometric hydroxyapatite. The specific surfaces of products were measured by BET method. The volume of micro- and mesopores was determined.

MeSH terms

  • Animals
  • Biocompatible Materials / chemistry
  • Biocompatible Materials / isolation & purification
  • Bone and Bones / chemistry*
  • Bone and Bones / ultrastructure
  • Calcium / analysis
  • Cattle
  • Durapatite / chemistry
  • Durapatite / isolation & purification*
  • Hot Temperature
  • Microscopy, Electron, Scanning
  • Phosphorus / analysis
  • Spectroscopy, Fourier Transform Infrared
  • Swine
  • X-Ray Diffraction

Substances

  • Biocompatible Materials
  • Phosphorus
  • Durapatite
  • Calcium