Genetic tool development for a new host for biotechnology, the thermotolerant bacterium Bacillus coagulans

Appl Environ Microbiol. 2010 Jun;76(12):4085-8. doi: 10.1128/AEM.03060-09. Epub 2010 Apr 16.

Abstract

Bacillus coagulans has good potential as an industrial production organism for platform chemicals from renewable resources but has limited genetic tools available. Here, we present a targeted gene disruption system using the Cre-lox system, development of a LacZ reporter assay for monitoring gene transcription, and heterologous d-lactate dehydrogenase expression.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacillus / genetics*
  • Biotechnology / methods*
  • Gene Expression Profiling / methods
  • Gene Knockout Techniques / methods
  • Genes, Reporter
  • Genetic Engineering / methods*
  • Genetics, Microbial / methods*
  • Lactate Dehydrogenases / genetics
  • Lactate Dehydrogenases / metabolism
  • Molecular Sequence Data
  • Recombinant Proteins / genetics
  • Recombinant Proteins / metabolism
  • beta-Galactosidase / genetics
  • beta-Galactosidase / metabolism

Substances

  • Recombinant Proteins
  • Lactate Dehydrogenases
  • D-lactate dehydrogenase
  • beta-Galactosidase

Associated data

  • GENBANK/GU323910
  • GENBANK/GU323911
  • GENBANK/GU323912
  • GENBANK/GU323913
  • GENBANK/GU323914
  • GENBANK/GU323915
  • GENBANK/GU323916
  • GENBANK/GU323917
  • GENBANK/GU323918