Abstract
Human teratocarcinoma cells (Tera-2) deprived of serum undergo programmed cell death which can be counteracted by simultaneous addition of IGF-II. This protective effect of IGF-II was specific in the sense that addition of IGF-binding protein 7 (IGFBP-7) resulted in an increased apoptotic rate almost comparable to that of the classical IGFBPs. Autoradiographic analysis of incorporated tritiated thymidine indicated that the proportion of S-phase cells was comparable, irrespective of total cell numbers. This further suggests that IGF-II rescues cells from apoptosis and that IGFBP-7 is a specific antagonist.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Apoptosis / drug effects*
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Autoradiography
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Cell Growth Processes / drug effects
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Cell Growth Processes / physiology
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Culture Media, Serum-Free
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Humans
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Insulin-Like Growth Factor Binding Protein 2 / pharmacology
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Insulin-Like Growth Factor Binding Proteins / pharmacology*
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Insulin-Like Growth Factor II / pharmacology
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Recombinant Proteins / pharmacology
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Teratocarcinoma / pathology*
Substances
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Culture Media, Serum-Free
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Insulin-Like Growth Factor Binding Protein 2
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Insulin-Like Growth Factor Binding Proteins
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Recombinant Proteins
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insulin-like growth factor binding protein-related protein 1
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Insulin-Like Growth Factor II