Insights into multienzyme docking in hybrid PKS-NRPS megasynthetases revealed by heterologous expression and genetic engineering

Chembiochem. 2010 May 17;11(8):1069-75. doi: 10.1002/cbic.201000103.
No abstract available

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Bacteria / enzymology
  • Bacteria / genetics*
  • Bacterial Proteins / chemistry
  • Bacterial Proteins / genetics
  • Bacterial Proteins / metabolism*
  • Escherichia coli / enzymology
  • Escherichia coli / genetics
  • Gene Expression
  • Methacrylates / metabolism
  • Models, Molecular
  • Molecular Sequence Data
  • Peptide Synthases / chemistry
  • Peptide Synthases / genetics
  • Peptide Synthases / metabolism*
  • Polyketide Synthases / chemistry
  • Polyketide Synthases / genetics
  • Polyketide Synthases / metabolism*
  • Protein Engineering*
  • Pseudomonas putida / enzymology
  • Pseudomonas putida / genetics
  • Sequence Alignment
  • Stigmatella aurantiaca / enzymology
  • Stigmatella aurantiaca / genetics
  • Thiazoles / metabolism

Substances

  • Bacterial Proteins
  • Methacrylates
  • Thiazoles
  • myxothiazol
  • Polyketide Synthases
  • Peptide Synthases
  • non-ribosomal peptide synthase