The authors present an experimental project that aims to establish an effective navigation system in accordance with European Council Regulation 1/2005 concerning animal welfare during transport. The prototype created during the project consists of both hardware and software components. An onboard unit is installed at truck level. It collects and transmits real-time information of the animal transport to a remote receiver database. A Web/geographic information system (GIS) application is used to analyse and monitor the information received. The architecture of the hardware and software of the project is presented, focusing on the features of the Web-GIS application.