A novel prism-chamber assembly was prepared for application in optical waveguide based chemical and biological sensors, making the sensor easily and reproducibly operate. By using the prism-chamber assembly, the performance of a composite waveguide based integrated Young interferometer sensor was investigated. The temporal interference pattern detected with a single-slit photodetector heavily relies on the slit width, and regular high-contrast patterns can be obtained under the condition that the slit width is smaller than the spatial periodicity of the sensor. Increasing the temperature of water in the chamber leads to a quasi-linear variation in the phase difference with Deltaphi/DeltaT approximately -9.1 degrees/degrees C. Significant dependence of the sensor's sensitivity on the polarization state of the guided mode was also observed. The sensor is stable and reliable, capable of real-time detection of very slow bioreactions at the interface.