The aim of this study was to report the prevalence of uropathogens and the frequency of extended spectrum beta-lactamases (ESBL) producing strains isolated from urine of outpatients in Primorsko-Goranska County in Croatia. We have retrospectively analyzed the results of 44, 321 urine cultures from January 01, 2008 till June 30, 2009. The study showed that ESBL production was confirmed in 189 (1.8%) of the total of 10,757 isolates. Rates of ESBL-producing isolates were 19%, 0,6%, and 5.2% for Klebisella pneumoniae, Escherichia coli and Proteus mirabillis, respectively. Geographic variations in pathogen occurrence and susceptibility profiles require continuous monitoring to provide information to guide the empiric therapeutic options.