Isolation of outer membrane proteins (OMP) from Salmonella cells using zwitterionic detergent and their separation by two-dimensional electrophoresis (2-DE)

Pol J Microbiol. 2009;58(4):363-6.

Abstract

We report that using the zwitterionic detergent Zwittergent Z 3-14 to isolate outer membrane proteins (OMPs) from Salmonella 048 is suitable for their separation by two-dimensional electrophoresis (2-DE) in a capillary tube system. Sample preparation is a very crucial step for any bacterial proteomic study. Some modifications were introduced to the 2-DE protocol suggested by O'Farrell and BioRad, which significantly impaired the resolution of proteins. 2-DE analysis of OMPs may be helpful in the interpretation of the variable susceptibility of Salmonella 048 rods to the bactericidal activity of serum.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Outer Membrane Proteins / chemistry
  • Bacterial Outer Membrane Proteins / metabolism*
  • Detergents / chemistry*
  • Electrophoresis, Gel, Two-Dimensional*
  • Salmonella / cytology*
  • Salmonella / metabolism*

Substances

  • Bacterial Outer Membrane Proteins
  • Detergents