Determination of beta-agonists in pig feed, pig urine and pig liver using capillary electrophoresis with electrochemical detection

Meat Sci. 2010 Jun;85(2):302-5. doi: 10.1016/j.meatsci.2010.01.018. Epub 2010 Feb 1.

Abstract

The fast separation capability of a capillary electrophoresis with electrochemical detection (CE-ED) system was demonstrated by determining five beta-agonists including clenbuterol, metoprolol, ractopamine, isoxsuprine and salbutamol in several real samples. Factors influencing the separation and detection were examined and optimized. Under the optimum conditions, the five beta-agonists could be well separated within 15min at a separation voltage of 14kV in a 100mmoL H(3)BO(3)-Na(2)B(4)O(7) running buffer (pH 9.0). This method was successfully used in the analysis of clenbuterol, metoprolol, ractopamine, isoxsuprine and salbutamol in pig feed, pig urine and pig liver, providing a useful method for monitoring the illegal use of beta-agonists from growth promotion in food-producing animals.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adrenergic beta-Agonists / chemistry*
  • Adrenergic beta-Agonists / urine*
  • Animals
  • Buffers
  • Drug Residues / analysis*
  • Electrochemical Techniques / veterinary*
  • Electrophoresis, Capillary / methods
  • Electrophoresis, Capillary / veterinary*
  • Hydrogen-Ion Concentration
  • Liver / chemistry*
  • Molecular Structure
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Swine*

Substances

  • Adrenergic beta-Agonists
  • Buffers