Abstract
Bombyx mori nucleopolyhedrovirus (BmNPV) ORF109 (Bm109) is a gene present in many lepidopteran NPVs, but its function is unknown. In this study, Bm109 was characterized. The transcript of Bm109 was detected at 12-72 h postinfection (p.i.). Polyclonal antiserum raised to a His-BM109 fusion protein recognized BM109 in infected cell lysates from 24 to 72 h p.i., suggesting that Bm109 is a late gene. Localization of the BM109 in BV and ODV by western analyses demonstrated that BM109 was proteins of ODV.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Bombyx / virology*
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DNA, Viral / genetics
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Gene Amplification
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Gene Expression Regulation, Viral
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Genes, Viral
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Mass Spectrometry
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Molecular Sequence Data
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Nucleopolyhedroviruses / genetics*
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Open Reading Frames*
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Polymerase Chain Reaction
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Sequence Alignment
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Sequence Analysis, Protein
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Sequence Homology, Amino Acid
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Viral Structural Proteins / analysis
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Viral Structural Proteins / chemistry
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Viral Structural Proteins / genetics*
Substances
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DNA, Viral
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Viral Structural Proteins