Characterization of Bombyx mori nucleopolyhedrovirus ORF109 that encodes a 25-kDa structural protein of the occlusion-derived virion

Curr Microbiol. 2010 Nov;61(5):451-7. doi: 10.1007/s00284-010-9637-y. Epub 2010 Apr 6.

Abstract

Bombyx mori nucleopolyhedrovirus (BmNPV) ORF109 (Bm109) is a gene present in many lepidopteran NPVs, but its function is unknown. In this study, Bm109 was characterized. The transcript of Bm109 was detected at 12-72 h postinfection (p.i.). Polyclonal antiserum raised to a His-BM109 fusion protein recognized BM109 in infected cell lysates from 24 to 72 h p.i., suggesting that Bm109 is a late gene. Localization of the BM109 in BV and ODV by western analyses demonstrated that BM109 was proteins of ODV.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Bombyx / virology*
  • DNA, Viral / genetics
  • Gene Amplification
  • Gene Expression Regulation, Viral
  • Genes, Viral
  • Mass Spectrometry
  • Molecular Sequence Data
  • Nucleopolyhedroviruses / genetics*
  • Open Reading Frames*
  • Polymerase Chain Reaction
  • Sequence Alignment
  • Sequence Analysis, Protein
  • Sequence Homology, Amino Acid
  • Viral Structural Proteins / analysis
  • Viral Structural Proteins / chemistry
  • Viral Structural Proteins / genetics*

Substances

  • DNA, Viral
  • Viral Structural Proteins