A synthesized GFP analogue emits via a hydrogen-bonding system

Chem Commun (Camb). 2010 Apr 28;46(16):2868-70. doi: 10.1039/b927461h. Epub 2010 Mar 5.

Abstract

Two GFP analogues o-HBDI and o-HBMO have been designed and synthesized. The former shows no fluorescence while the latter shows strong fluorescence due to the formation of an intramolecular hydrogen-bond. At low temperatures, both molecules have strong fluorescence.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Benzylidene Compounds / chemistry*
  • Green Fluorescent Proteins / chemical synthesis*
  • Green Fluorescent Proteins / chemistry
  • Hydrogen Bonding
  • Imidazoles / chemical synthesis
  • Imidazoles / chemistry*
  • Oxazoles / chemical synthesis*
  • Oxazoles / chemistry
  • Spectrometry, Fluorescence
  • Temperature

Substances

  • 4-(2-hydroxybenzylidene)-1,2-dimethyl-1H-imidazol-5(4H)-one
  • 4-(2-hydroxybenzylidene)-2-methyloxazol-5(4H)-one
  • Benzylidene Compounds
  • Imidazoles
  • Oxazoles
  • Green Fluorescent Proteins