Abstract
Two GFP analogues o-HBDI and o-HBMO have been designed and synthesized. The former shows no fluorescence while the latter shows strong fluorescence due to the formation of an intramolecular hydrogen-bond. At low temperatures, both molecules have strong fluorescence.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Benzylidene Compounds / chemistry*
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Green Fluorescent Proteins / chemical synthesis*
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Green Fluorescent Proteins / chemistry
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Hydrogen Bonding
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Imidazoles / chemical synthesis
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Imidazoles / chemistry*
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Oxazoles / chemical synthesis*
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Oxazoles / chemistry
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Spectrometry, Fluorescence
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Temperature
Substances
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4-(2-hydroxybenzylidene)-1,2-dimethyl-1H-imidazol-5(4H)-one
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4-(2-hydroxybenzylidene)-2-methyloxazol-5(4H)-one
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Benzylidene Compounds
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Imidazoles
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Oxazoles
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Green Fluorescent Proteins