Objective: To establish a method for the determination of streptomycin in honey by determined hydrophilic interaction chromatography combined with tandem mass spectrometry (HILIC-MS/MS).
Methods: A hilic column was used, and the mobile phase was consisted of acetonitrile (5 mmol/L ammonium acetate and 0.1% formic acid, 60:40). Samples were extracted by the cation-exchange SPE cartridge. The extraction samples were then identified and quantitated by HILIC-ESI-MS/MS.
Results: Linear calibration curves were obtained at the concentration ranges from 10. 5 microg/kg to 105 microg/kg (the correlation coefficients were above 0.99). The limit of detection was 1.05 microg/kg. The average recoveries for streptomycin spiked in honey ranged from 71.3 to 82.5% and their relative standard were between 3.4% and 8.2%.
Conclusion: The method for determination of streptomycin in honey could be simple, sensitive and accurate.