2-C-methyluridine modified hammerhead ribozyme against the estrogen receptor

Bioorg Med Chem Lett. 2010 May 1;20(9):2806-8. doi: 10.1016/j.bmcl.2010.03.060. Epub 2010 Mar 17.

Abstract

A new synthesis of 2'-C-methyluridine phosphoramidite is presented. Special emphasis is dedicated to the improvement of the protection of the tertiary 2'-hydroxyl group. Comparison to previous protecting strategies and analysis of stability under 5'-DMTr removing conditions are discussed. The synthetic incorporation of this modified nucleoside into the catalytic core of a hammerhead ribozyme against the estrogen receptor alpha protein (ER-alpha), and transfection experiments in MCF-7 cell line are also presented.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cell Line, Tumor
  • Humans
  • Oligonucleotides / chemistry
  • RNA, Catalytic / chemistry*
  • RNA, Catalytic / metabolism
  • RNA, Messenger / metabolism
  • Receptors, Estrogen / metabolism*
  • Uridine / analogs & derivatives*
  • Uridine / chemical synthesis
  • Uridine / chemistry

Substances

  • 2'-C-methyluridine
  • Oligonucleotides
  • RNA, Catalytic
  • RNA, Messenger
  • Receptors, Estrogen
  • hammerhead ribozyme
  • locked nucleic acid
  • Uridine