Abstract
The MADS-domain transcription factor APETALA1 (AP1) is a key regulator of Arabidopsis flower development. To understand the molecular mechanisms underlying AP1 function, we identified its target genes during floral initiation using a combination of gene expression profiling and genome-wide binding studies. Many of its targets encode transcriptional regulators, including known floral repressors. The latter genes are down-regulated by AP1, suggesting that it initiates floral development by abrogating the inhibitory effects of these genes. Although AP1 acts predominantly as a transcriptional repressor during the earliest stages of flower development, at more advanced stages it also activates regulatory genes required for floral organ formation, indicating a dynamic mode of action. Our results further imply that AP1 orchestrates floral initiation by integrating growth, patterning, and hormonal pathways.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Arabidopsis / genetics*
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Arabidopsis / growth & development*
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Arabidopsis / metabolism
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Arabidopsis Proteins / genetics
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Arabidopsis Proteins / metabolism*
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Binding Sites
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Chromatin Immunoprecipitation
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Down-Regulation
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Flowers / growth & development*
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Gene Expression Profiling
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Gene Expression Regulation, Plant*
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Genes, Plant
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Genome, Plant
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Homeodomain Proteins / genetics
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Homeodomain Proteins / metabolism
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MADS Domain Proteins / genetics
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MADS Domain Proteins / metabolism*
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Oligonucleotide Array Sequence Analysis
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Transcription Factors / genetics
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Transcription Factors / metabolism*
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Transcription Initiation Site
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Transcription, Genetic*
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Transcriptional Activation
Substances
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AP1 protein, Arabidopsis
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Arabidopsis Proteins
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Homeodomain Proteins
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MADS Domain Proteins
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SEP3 protein, Arabidopsis
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Transcription Factors
Associated data
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GEO/GSE20138
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GEO/GSE20176
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GEO/GSE20184