Quadruplex real-time quantitative PCR assay for the detection of pathogens related to late-onset ventilator-associated pneumonia: a preliminary report

Merab M Rios-Licea; Francisco J Bosques; Alejandro C Arroliga; Juan O Galindo-Galindo; Elvira Garza-Gonzalez

doi:10.1016/j.mimet.2010.03.018

Quadruplex real-time quantitative PCR assay for the detection of pathogens related to late-onset ventilator-associated pneumonia: a preliminary report

J Microbiol Methods. 2010 Jun;81(3):232-4. doi: 10.1016/j.mimet.2010.03.018. Epub 2010 Mar 28.

Authors

Merab M Rios-Licea¹, Francisco J Bosques, Alejandro C Arroliga, Juan O Galindo-Galindo, Elvira Garza-Gonzalez

Affiliation

¹ Departamento de Microbiología, Facultad de Medicina, Universidad Autónoma de Nuevo León, Mexico.

PMID: 20353803
DOI: 10.1016/j.mimet.2010.03.018

Abstract

A quadruplex real-time (RT) qPCR assay for the detection and quantification in 4h of Staphylococcusaureus, Pseudomonasaeruginosa, Acinetobacterbaumannii and Stenotrophomonasmaltophilia directly from bronchoalveolar lavage specimens was developed. The specificity of the assay was 100% for all four species.

Publication types

Evaluation Study

MeSH terms

Adolescent
Adult
Aged
Aged, 80 and over
Bacteriological Techniques / methods*
Bronchoalveolar Lavage Fluid / microbiology
Female
Gram-Negative Bacteria / isolation & purification
Gram-Negative Bacterial Infections / diagnosis*
Gram-Negative Bacterial Infections / microbiology
Gram-Positive Bacteria / isolation & purification
Gram-Positive Bacterial Infections / diagnosis*
Gram-Positive Bacterial Infections / microbiology
Humans
Male
Middle Aged
Pneumonia, Ventilator-Associated / diagnosis*
Pneumonia, Ventilator-Associated / microbiology
Polymerase Chain Reaction / methods*
Sensitivity and Specificity
Young Adult