Benomyl-resistant mutant strain of Trichoderma sp. with increased mycoparasitic activity

Folia Microbiol (Praha). 2010 Jan;55(1):102-4. doi: 10.1007/s12223-010-0016-7. Epub 2010 Mar 25.

Abstract

Application of UV radiation to the strain Trichoderma sp. T-bt (isolated from lignite) resulted in the T-brm mutant which was resistant to the systemic fungicide benomyl. The tub2 gene sequence in the T-brm mutant differed from the parent as well as the collection strain (replacing tyrosine with histidine in the TUB2 protein). Under in vitro conditions this mutant exhibited a higher mycoparasitic activity toward phytopathogenic fungi.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Substitution
  • Antifungal Agents / pharmacology*
  • Benomyl / pharmacology*
  • Fungal Proteins / genetics
  • Microbial Sensitivity Tests
  • Mutation, Missense
  • Trichoderma / drug effects*
  • Trichoderma / physiology*
  • Trichoderma / radiation effects
  • Ultraviolet Rays
  • Virulence

Substances

  • Antifungal Agents
  • Fungal Proteins
  • Benomyl