An indirect ELISA for serodiagnosis of cattle footrot caused by Fusobacterium necrophorum

Anaerobe. 2010 Aug;16(4):317-20. doi: 10.1016/j.anaerobe.2010.03.007. Epub 2010 Mar 19.

Abstract

A serodiagnostic ELISA (rL-ELISA) using recombinant truncated leukotoxin protein PL2 (aa 311-644) of Fusobacterium necrophorum as antigen was developed for detection of antibodies against F. necrophorum from cattle footrot. In rL-ELISA, the recombinant diagnostic antigen showed no cross-reaction with antisera against bovine foot and mouth disease virus, bovine rhinotracheitis virus, bovine viral diarrhea virus, bovine rotavirus type A, bovine Escherichia coli, and bovine Salmonella. The rL-ELISA could confirm the existence of antibodies against F. necrophorum at day 7 after infection. Detection of the field samples indicated relative sensitivity of rL-ELISA to nL-ELISA using the purified native leukotoxin A as antigen was 96.43%, and relative specificity of rL-ELISA to nL-ELISA was 94.26%. These data demonstrated the rL-ELISA would have a potential use for early diagnosis of cattle footrot caused by F. necrophorum.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Bacterial / blood*
  • Bacterial Toxins
  • Cattle
  • Cattle Diseases / diagnosis*
  • Cattle Diseases / microbiology
  • Enzyme-Linked Immunosorbent Assay / methods*
  • Exotoxins*
  • Fusobacterium Infections / diagnosis
  • Fusobacterium Infections / microbiology
  • Fusobacterium Infections / veterinary*
  • Fusobacterium necrophorum / immunology
  • Fusobacterium necrophorum / isolation & purification*
  • Recombinant Proteins
  • Sensitivity and Specificity
  • Serologic Tests / methods
  • Time Factors

Substances

  • Antibodies, Bacterial
  • Bacterial Toxins
  • Exotoxins
  • Recombinant Proteins
  • leukotoxin