Abstract
Platensimycin (1) and platencin (2) are novel antibiotic leads against multidrug resistant pathogens. The production of 2 in Streptomyces platensis MA7339 is under the control of ptnR1, a GntR-like transcriptional regulator. Inactivating ptnR1 afforded S. platensis MA7339 mutant strain SB12600 that overproduces 2 at a titer approximately 100-fold greater than that from the wild-type strain and accumulates platencin A(1) (3) and eight new congeners, platencins A(2)-A(9) (4-11). The isolation, structural elucidation, and antibacterial activity of 4-11, in comparison to 1-3, are described.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Aminophenols / chemistry*
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Aminophenols / isolation & purification
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Aminophenols / metabolism*
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Aminophenols / pharmacology
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Anti-Bacterial Agents / biosynthesis*
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Anti-Bacterial Agents / chemistry*
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Anti-Bacterial Agents / isolation & purification
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Anti-Bacterial Agents / pharmacology
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Genetic Engineering / methods*
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Microbial Sensitivity Tests
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Polycyclic Compounds / chemistry*
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Polycyclic Compounds / isolation & purification
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Polycyclic Compounds / metabolism*
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Polycyclic Compounds / pharmacology
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Staphylococcus aureus / drug effects
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Streptomyces / genetics*
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Streptomyces / metabolism*
Substances
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Aminophenols
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Anti-Bacterial Agents
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Polycyclic Compounds
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platencin